difco macconkey agar base (Difco)

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Difco difco macconkey agar base

(A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and <t>MacConkey</t> maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.

Difco Macconkey Agar Base, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macconkey+agar+difco/agar+macconkey+%EB%85%B9%EC%83%89%ED%95%A9%EC%84%B1+%EB%B3%B5%ED%95%A9%EB%AC%BC%EC%A7%88+%EC%84%B1+%EC%9C%84%ED%95%B4+%EC%9D%80%EB%82%98%EB%85%B8+%EC%A7%80%EC%9D%98+%EC%BD%94%ED%8C%85+%ED%82%A4%ED%86%A0%EC%82%B0+%ED%8F%89%EA%B0%80%EB%A5%BC+%ED%95%AD%EA%B7%A0/pmc12952977-46-0-0
Average 86 stars, based on 1 article reviews

difco macconkey agar base - by Bioz Stars, 2026-09

86/100 stars

Images

1) Product Images from "A prophage-expressed type IV pilus component provides anti-phage defense"

Article Title: A prophage-expressed type IV pilus component provides anti-phage defense

Journal: Cell reports

doi: 10.1016/j.celrep.2025.116759

Figure Legend Snippet: (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and MacConkey maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.

Techniques Used: Expressing, Fluorescence, Microscopy, Immunolabeling, Labeling

Incubation:

Article Title: Resistome phylodynamics of multidrug-resistant Shigella isolated from diarrheal patients
Article Snippet: .. Standard microbiological culture methods like the aerobic culture on MacConkey agar (Difco) and subsequent 18-h incubation at 37°C were used to isolate the MDR- Shigella strains ( ). .. The 11 Shigella strains (4 S . flexneri , 4 S . sonnei, and 3 S . boydii ) were serologically confirmed by slide agglutination tests applying commercially available antiserum kits (Denka Seiken, Tokyo, Japan) ( ).

Article Title: Food hygiene practices and associated factors among food handlers in food establishments of Jimma City, Southwest Ethiopia.
Article Snippet: .. Whereas for the confinement of gram-negative microscopic organisms, swabs obtained from the hands of participants were refined onto MacConkey agar (Difco), Salmonella-Shigella agar (SSA) (Oxoid), and after that was incubated at 37°C for 24 hours. ..

Article Title: Whole-genome-based characterization of Escherichia albertii strains isolated from paediatric diarrhoeal cases in Kolkata, India
Article Snippet: .. Faecal specimens were inoculated on MacConkey agar (Difco, USA) and incubated at 37 °C for 16–18 h. If available, three typical non-lactose fermenting colonies per sample from the MacConkey agar plate were picked and subcultured on Luria Bertani agar (LBA; Difco, USA). ..

Article Title: Whole-genome-based characterization of Escherichia albertii strains isolated from paediatric diarrhoeal cases in Kolkata, India.
Article Snippet: .. Faecal specimens were inoculated on MacConkey agar (Difco, USA) and incubated at 37 °C for 16–18 h. If available, three typical non- lactose fermenting colonies per sample from the MacConkey agar plate were picked and subcultured on Luria Bertani agar (LBA; Difco, USA). ..

Article Title: Resistome phylodynamics of multidrug-resistant Shigella isolated from diarrheal patients.
Article Snippet: .. Standard microbiological culture methods like the aerobic culture on MacConkey agar (Difco) and subsequent 18-h incubation at 37°C were used to isolate the MDR-Shigella strains (36). .. The 11 Shigella strains (4 S. flexneri, 4 S. sonnei, and 3 S. boydii) were serologically confirmed by slide agglutination tests applying commercially available antiserum kits (Denka Seiken, Tokyo, Japan) (37).

other:

Article Title: Meat ducks as carriers of antimicrobial-resistant Escherichia coli harboring transferable R plasmids.
Article Snippet: Briefly, the cloacal swab samples were directly inoculated on MacConkey agar (Difco; BD, USA).

Starch:

Article Title: Herbicide-treated soil as a reservoir of beneficial bacteria: microbiome analysis and PGP bioinoculants in maize
Article Snippet: .. From the second batch, serial dilutions were plated on SCA (Starch Casein Agar, HiMedia Laboratories, Mumbai, India) supplemented with 150 μg/mL nalidixic acid to support the growth of Gram-positive bacteria or on MacConkey agar (Difco, Detroit, MI, USA) to support the growth of Gram-negative bacteria. ..

Bacteria:

Article Title: Supplementation of protease with low protein diets improves incidence of frequency and nitrogen metabolism in weaned pigs
Article Snippet: .. To measure the number of Lactobacillus and Escherichia coli ( E. coli ) bacteria in feces by experimental treatment, de man, rogosa and sharpe (MRS) agar was used for Lactobacillus and MacConkey agar (Difco, Tucker, GA, USA) was used for E. coli . ..

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